Tropic viruses

To analyze the basis for this difference, we used the HeLa-CD4 panel to compare a molecularly cloned T-cell-tropic PR virus ELI1 with six of its variants that grow to different extents in CD4-positive leukemic cell lines and that differ only at specific positions in their gp and gp41 envelope glycoproteins.

All mutations in gp or gp41 that contributed to laboratory adaptation preferentially enhanced infectivity for cells that had little CD4 and thereby decreased the CD4 dependencies of the infections. There was a close correlation between abilities of T-cell-tropic ELI viruses to grow in an expanded repertoire of leukemic cell lines, the reduced CD4 dependencies of their infections of the HeLa-CD4 panel, and their sensitivities to inactivation by soluble CD4 sCD4.

Since all of the ELI viruses can efficiently use CXCR-4 as a coreceptor, we conclude that an increase in viral affinity for CD4 rather than a switch in coreceptor specificity is principally responsible for laboratory adaption of T-cell-tropic HIV Syncytium-inducing activities of the ELI viruses, especially when analyzed on cells with low amounts of CD4, were also highly correlated with their laboratory-adapted properties.

Biochemical Typing of Murine Viral Proteins. Nucleic acid homology of murine type-C viral genes. Presence of murine leukemia virus envelope proteins gp70 and p15 E in a common polyprotein of infected cells. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. Avian leukemia-sarcoma viruses. Structural markers on core protein p30 of murine leukemia virus: functional correlation with Fv-1 tropism.

Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures.

Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Mammalian leukemia-sarcoma viruses.

G RADA1 : a new cell surface antigen of mouse leukemia defined by naturally occurring antibody and its relationship to murine leukemia virus. J Exp Med. Multiplicity-dependent kinetics and murine leukemia virus infection in Fvsensitive and Fvresistant cells. Induction of GIX antigen and gross cell surface antigen after infection by ecotropic and xenotropic murine leukemia viruses in vitro.

Polymorphism of endogenous murine leukemia viruses revealed by isoelectric focusing in polyacrylamide gels. Phenotypic mixing between N- and B-tropic murine leukemia viruses: infectious particles with dual sensitivity to Fv-1 restriction. Segregation of genetic information for a B-tropic leukemia virus with the structural locus for BALB:virus Evidence for recombination between N- and B-tropic murine leukemia viruses: analysis of three virion proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Type C viruses of baboons: isolation from normal cell cultures. High frequency of aberrant expression of Moloney murine leukemia virus in clonal infections. Characterization of molecular species carrying gross cell surface antigen. Isolation of temperature-sensitive mutants of murine leukemia virus. Structural proteins of oncogenic ribonucleic acid viruses. Interspec II, a new interspecies antigen.

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